Corticosteroids

The chemical synthesis of naturally occurring corticosteroids has led to the generation of artificial corticosteroids that are used in therapeutic veterinary drugs.  Many of these drugs are also used illegally as growth promoters particularly in cattle, often in combination with J-agonists.  Dexamethasone, Flumethasone and Betamethasone all belong to this group of drugs that are also known as glucocorticosteriods. The European Union has banned the use of corticosteroids administration for fattening purposes in animals.

The Randox Food Diagnostics Corticosteroids ELISA test kit offers excellent limits of detection for the screening of growth promoter residues across a range of food and veterinary matrices.

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No of Tests

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DM2156

96 tests

1

POA

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Corticosteroids ELISA

Assay

Compound

% Cross Reactivity

LOD (ppb)

Corticosteroid



Dexamethasone

100

Tissue 0.25

Urine 2.5

Urine (IAC) 0.1

Serum  0.1



Dexamethasone 21 Acetate

162

Dexamethasone 21 Phospate

3.6

Flumethasone

Flumethasone

50

Tissue 0.5

Urine 5.0

Urine (IAC) 0.2

Serum 0.2

 

Betamethasone







Betamethasone

43

Tissue 0.6 

Urine 5.8

Urine (IAC) 0.25 

Serum 0.23







Betamethasone 21 Acetate

73

Betamethasone 21 Phosphate

2.0

Triamcionlone

1.0

Prednisolone

1.7

Cortisol

<0.5

Cortisone

<0.5

Detection of multiple compounds

  • Simultaneously screen for flumethasone, dexamethasone and betamethasone

Very low cross-reactivity for cortisol (<0.5%)

  • Minimising interference from endogenous cortisol in urine samples

Rapid and simple assay format

  • 40 samples can be analysed in 90 mins, less labour
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