Quinolones

Quinolones are a group of synthetic antimicrobials that inhibit the activity of bacterial DNA gyrase enzymes. The earliest members of this group of compounds are active against gram-negative bacteria. The newer fluoroquinolone antibiotics, a fluorine-containing subclass of the quinolones, have been found to have enhanced antibacterial activity against gram-negative bacteria and they are also active against gram-positive bacteria. The main application of quinolones is the treatment of infection, including, gastrointestinal and respiratory infections both in human and veterinary clinical practices. However, the presence of residues of these compounds in food producing animals has to be controlled as their widespread use can lead to the emergence of antibiotic resistant bacteria. As a result maximum residue limits have been set for several quinolones depending on the animal species and matrices.

The Randox Food Diagnostics Quinolones ELISA test kit detects 17 Quinolone analytes in milk and tissue samples.

Catalogue No_

No of Tests

Quantity

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QL3454

96 tests

1

POA

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Quinolones ELISA

Assay

Compound

% Cross-Reactivity

LOD (ppb)

Quinolones

Nadifloxacin

429

Tissue 1.4

Milk 0.06

Rufloxacin

401

Danofloxacin

324

Enrofloxacin

300

Ciprofloxacin

166

Levofloxacin

138

Ofloxacin

120

Pefloxacin

120

Prulifloxacin

109

Norfloxacin

100

Difloxacin

65

Sarafloxacin

59

Enoxacin

59

Marbofloxacin

43

Fleroxacin

34

Clinafloxacin

31

Lomefloxacin

31

Balofloxacin

14

Orbifloxacin

14

Oxolinic acid

10

Gatifloxacin

9

Moxifloxacin

8

Tosufloxacin

8

Pazufloxacin

3

Pipemidic acid

3

Flumequine

1

Sparfloxacin

1

Cinoxacin

<0.3

Nalidixic acid

<0.1

Detects 17 Quinolones analytes

  • Detects multiple compounds from a single sample

Simple sample preparations

  • Simple to use with little technical expertise required

Plates pre-coated with analyte specific antibody

  • Reducing assay set up time
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